ELISA/assay

Human Interleukin 1 Racp ELISA Kit

MBS720693

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Interleukin 1 Racp ELISA Kit

Interleukin 1 Racp

interleukin-1 receptor accessory protein isoform 2; Interleukin-1 receptor accessory protein; interleukin-1 receptor accessory protein; IL-1R3; interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin-1 receptor accessory protein beta; interleukin 1 receptor accessory protein; Interleukin-1 receptor 3; IL-1R-3; IL-1R3

IL 1Racp

IL1RAP; IL1RAP; IL1R3; C3orf13; IL-1RAcP; C3orf13; IL1R3; IL-1 receptor accessory protein; IL-1RAcP; IL-1R-3; IL-1R3

IL1AP_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of IL-1Racp. No significant cross-reactivity or interference between IL-1Racp and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-1Racp and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This IL-1Racp ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL-1Racp. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-1Racp ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-1Racp. Standards or samples are then added to the microtiter plate wells and IL-1Racp if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-1Racp present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-1Racp are added to each well to "sandwich" the IL-1Racp immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-1Racp and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1Racp concentration in each sample is interpolated from this standard curve.

268840341

NP_001161402.1

NM_001167930.1

78,603 Da

Apoptosis Pathway (83060); Apoptosis Pathway (470); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL-1 Signaling Pathway (198908); Immune System Pathway (106386); Inflammatory Mediator Regulation Of TRP Channels Pathway (948277); Inflammatory Mediator Regulation Of TRP Channels Pathway (948307); Interleukin-1 Signaling Pathway (160141)

Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes the interleukin 1 receptor accessory protein. The protein is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. [provided by RefSeq, Nov 2009]

IL1RAP: Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 3q28. Cellular Component: membrane; integral to plasma membrane; extracellular region; plasma membrane. Molecular Function: signal transducer activity; interleukin-1 receptor activity. Biological Process: interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; innate immune response; protein complex assembly; immune response; inflammatory response

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675