ELISA/assay

Rat Anoctamin-1 (ANO1) ELISA Kit

MBS7206898

48-Strip-Wells

440 USD

ELISA Kit

Rat Anoctamin-1 (ANO1) ELISA Kit

[Anoctamin-1 (ANO1)]

[anoctamin-1; Anoctamin-1; anoctamin-1; Ca2+-activated Cl- channel; oral cancer overexpressed 2; tumor-amplified and overexpressed sequence 2; discovered on gastrointestinal stromal tumors protein 1; transmembrane protein 16A (eight membrane-spanning domains); anoctamin 1, calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2]

[ANO1]

[ANO1; ANO1; DOG1; TAOS2; ORAOV2; TMEM16A; DOG1; ORAOV2; TAOS2; TMEM16A]

ANO1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of ANO1. No significant cross-reactivity or interference between ANO1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANO1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/ml

Cell Biology

Intended Uses: This ANO1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ANO1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay ANO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANO1 antibody and an ANO1-HRP conjugate. The assay sample and buffer are incubated together with ANO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANO1 concentration since ANO1 from samples and ANO1-HRP conjugate compete for the anti-ANO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANO1 from the sample, fewer sites are left to bind ANO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANO1 concentration in each sample is interpolated from this standard curve.

194306539

NP_060513.5

NM_018043.5

74,369 Da

Ion Channel Transport Pathway (187193); Stimuli-sensing Channels Pathway (771568); Transmembrane Transport Of Small Molecules Pathway (106572)

ANO1: Acts as a calcium-activated chloride channel. Required for normal tracheal development. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter; Membrane protein, multi-pass; Transporter, ion channel; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q13.3. Cellular Component: cytoplasm; apical plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity; calcium activated cation channel activity. Biological Process: regulation of membrane potential; multicellular organismal development; chloride transport; transmembrane transport; cation transport

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415