ELISA/assay

Human Adseverin (SCIN) ELISA Kit

MBS7206431

48-Strip-Wells

470 USD

ELISA Kit

Human Adseverin (SCIN) ELISA Kit

Adseverin (SCIN)

adseverin isoform 1; Adseverin; adseverin; scinderin; Scinderin

SCIN

SCIN; SCIN; KIAA1905

ADSV_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of SCIN. No significant cross-reactivity or interference between SCIN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCIN and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 mug/mL.

Intended Uses: This SCIN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SCIN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

. Principle of the assay: SCIN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SCIN antibody and an SCIN-HRP conjugate. The assay sample and buffer are incubated together with SCIN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCIN concentration since SCIN from samples and SCIN-HRP conjugate compete for the anti-SCIN antibody binding site. Since the number of sites is limited, as more sites are occupied by SCIN from the sample, fewer sites are left to bind SCIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCIN concentration in each sample is interpolated from this standard curve.

162951877

NP_001106177.1

NM_001112706.2

52,814 Da

Endochondral Ossification Pathway (198812); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Viral Carcinogenesis Pathway (658418); Viral Carcinogenesis Pathway (662842)

SCIN is a Ca(2+)-dependent actin-severing and -capping protein (Zunino et al., 2001 [PubMed 11568009]).[supplied by OMIM, May 2010]

SCIN: Ca(2+)-dependent actin filament-severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Regulates chondrocyte proliferation and differentiation. MAP kinases p38 and ERK1/2 mediate the adseverin-induced accelerated differentiation of non-hypertrophic chondrocytes. Belongs to the villin/gelsolin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7p21.3. Cellular Component: cytoskeleton; protein complex; cytoplasm; cell cortex. Molecular Function: actin filament binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylserine binding; calcium ion binding; actin binding; phosphatidylinositol binding. Biological Process: actin filament severing; negative regulation of cell proliferation; actin filament capping; positive regulation of apoptosis; positive regulation of secretion; positive regulation of megakaryocyte differentiation; regulation of chondrocyte differentiation; actin nucleation; calcium ion-dependent exocytosis

48-Strip-Wells

470 USD

96-Strip-Wells

675