ELISA/assay

Human Anti Neutrophil Elastase Antibody ELISA Kit

MBS720638

96 Strip Wells

675 USD

ELISA Kit

Human Anti Neutrophil Elastase Antibody ELISA Kit

Anti Neutrophil Elastase Antibody

neutrophil elastase, partial; Neutrophil elastase; neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase; elastase, neutrophil expressed; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase

ANEA

ELANE; ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; ELA2; HLE

ELNE_HUMAN

Human

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 1.0 mU/mL

Neurobiology

For Sample Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This NE-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NE-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . Principle of the Assay: NE-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing NE antigen and an NE-Ab-HRP conjugate. The assay sample and buffer are incubated together with NE-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NE-Ab concentration since NE-Ab from samples and NE-Ab-HRP conjugate compete for the NE antigen binding site. Since the number of sites is limited, as more sites are occupied by NE-Ab from the sample, fewer sites are left to bind NE-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NE-Ab concentration in each sample is interpolated from this standard curve.

386981

AAA36359.1

28,518 Da

Activation Of Matrix Metalloproteinases Pathway 576264!!C-MYB Transcription Factor Network Pathway 138073!!Collagen Degradation Pathway 730309!!Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262!!Systemic Lupus Erythematosus Pathway 83122!!Systemic Lupus Erythematosus Pathway 534!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987!!Amb2 Integrin Signaling Pathway 137945

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]

96 Strip Wells

675 USD