ELISA/assay

Human Natural resistance associated macrophage protein 1 (SLC11A1) ELISA Kit

MBS7206344

48-Strip-Wells

470 USD

ELISA Kit

Human Natural resistance associated macrophage protein 1 (SLC11A1) ELISA Kit

Natural resistance associated macrophage protein 1 (SLC11A1)

natural resistance associated macrophage protein 1; Natural resistance-associated macrophage protein 1; natural resistance-associated macrophage protein 1; NRAMP 1; solute carrier family 11 member 1; Solute carrier family 11 member 1

SLC11A1

SLC11A1; SLC11A1; NRAMP1; NRAMP1; NRAMP 1

NRAM1_PIG

Human

This assay has high sensitivity and excellent specificity for detection of SLC11A1. No significant cross-reactivity or interference between SLC11A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC11A1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This SLC11A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SLC11A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Priciple of the assay: SLC11A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SLC11A1 antibody and an SLC11A1-HRP conjugate. The assay sample and buffer are incubated together with SLC11A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC11A1 concentration since SLC11A1 from samples and SLC11A1-HRP conjugate compete for the anti-SLC11A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC11A1 from the sample, fewer sites are left to bind SLC11A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC11A1 concentration in each sample is interpolated from this standard curve.

3258616

AAC24491.1

59,012 Da

Lysosome Pathway (98759); Lysosome Pathway (96865); Mineral Absorption Pathway (212235); Mineral Absorption Pathway (212220)

48-Strip-Wells

470 USD

96-Strip-Wells

675