ELISA/assay

Human Eukaryotic Translation Initiation Factor 3A ELISA Kit

MBS720629

48-Strip-Wells

470 USD

ELISA Kit

Human Eukaryotic Translation Initiation Factor 3A ELISA Kit

Eukaryotic Translation Initiation Factor 3A

Eukaryotic translation initiation factor 3 subunit A; Eukaryotic translation initiation factor 3 subunit A; eukaryotic translation initiation factor 3 subunit A; eIF-3-theta; eukaryotic translation initiation factor 3 subunit 10; eukaryotic translation initiation factor 3, subunit 10 (theta); eukaryotic translation initiation factor 3, subunit A; Eukaryotic translation initiation factor 3 subunit 10; eIF-3-theta

eIF3A

Eif3a; Eif3a; ZH12; Eif3s10; Eif3s10; eIF3a

EIF3A_RAT

Human

This assay has high sensitivity and excellent specificity for detection of EIF-3A. No significant cross-reactivity or interference between EIF-3A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EIF-3A and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This EIF-3A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EIF-3A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: EIF-3A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EIF-3A antibody and an EIF-3A-HRP conjugate. The assay sample and buffer are incubated together with EIF-3A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EIF-3A concentration since EIF-3A from samples and EIF-3A-HRP conjugate compete for the anti-EIF-3A antibody binding site. Since the number of sites is limited, as more sites are occupied by EIF-3A from the sample, fewer sites are left to bind EIF-3A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EIF-3A concentration in each sample is interpolated from this standard curve.

229488252

Q1JU68.2

163,196 Da

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (1009712); Cap-dependent Translation Initiation Pathway (1009709); Eukaryotic Translation Initiation Pathway (1009708); Formation Of A Pool Of Free 40S Subunits Pathway (1009710); Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway (1009711); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (1009715); Gene Expression Pathway (1010398); L13a-mediated Translational Silencing Of Ceruloplasmin Expression Pathway (1009717); Metabolism Of Proteins Pathway (1009706); RNA Transport Pathway (177822)

eIF3-theta: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Interacts with EIF4G1. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2. Belongs to the eIF-3 subunit A family. Protein type: Translation; RNA-binding; Translation initiation. Cellular Component: eukaryotic translation initiation factor 3 complex; membrane; cytoplasm; nucleolus; nucleus. Molecular Function: translation initiation factor activity. Biological Process: formation of translation initiation complex; formation of translation preinitiation complex; regulation of translational initiation

48-Strip-Wells

470 USD

96-Strip-Wells

675