ELISA/assay

Mouse Pituitary adenylate cyclase activating polypeptide ELISA Kit

MBS720617

48-Strip-Wells

470 USD

ELISA Kit

Mouse Pituitary adenylate cyclase activating polypeptide ELISA Kit

Pituitary adenylate cyclase activating polypeptide

pituitary adenylate cyclase activating polypeptide; Pituitary adenylate cyclase-activating polypeptide; pituitary adenylate cyclase-activating polypeptide; prepro-PACAP; adenylate cyclase activating polypeptide 1 (pituitary); PRP-48

PACAP

ADCYAP1; ADCYAP1; PACAP; PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38

PACA_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of PACAP. No significant cross-reactivity or interference between PACAP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PACAP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This PACAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PACAP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: PACAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PACAP antibody and an PACAP-HRP conjugate. The assay sample and buffer are incubated together with PACAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PACAP concentration since PACAP from samples and PACAP-HRP conjugate compete for the anti-PACAP antibody binding site. Since the number of sites is limited, as more sites are occupied by PACAP from the sample, fewer sites are left to bind PACAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PACAP concentration in each sample is interpolated from this standard curve.

245806

AAB21470.1

18,835 Da

Activation Of TRKA Receptors Pathway (106456); Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Insulin Secretion Pathway (777534); NGF Signalling Via TRKA From The Plasma Membrane Pathway (106459); NGF-independant TRKA Activation Pathway (106458); SIDS Susceptibility Pathways (198901)

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 18p11. Cellular Component: extracellular space; extracellular region; terminal button. Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor signaling protein activity; pituitary adenylate cyclase-activating peptide receptor binding; pituitary adenylate cyclase activating polypeptide activity; receptor binding. Biological Process: behavioral fear response; nerve growth factor receptor signaling pathway; negative regulation of potassium ion transport; female pregnancy; positive regulation of vasodilation; sensory perception of pain; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell cycle; histamine secretion; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; regulation of protein localization; ovarian follicle development; cell-cell signaling; neuropeptide signaling pathway; positive regulation of cell proliferation; neurite development; negative regulation of acute inflammatory response to antigenic stimulus; adenylate cyclase activation; positive regulation of interleukin-6 production; ATP metabolic process; response to starvation; positive regulation of growth hormone secretion; response to ethanol; regulation of postsynaptic membrane potential; pituitary gland development; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of acute inflammatory response to non-antigenic stimulus; regulation of G-protein coupled receptor protein signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

48-Strip-Wells

470 USD

96-Strip-Wells

675