ELISA/assay

Rat 14-3-3 protein gamma (YWHAG) ELISA Kit

MBS7205662

48-Strip-Wells

470 USD

ELISA Kit

Rat 14-3-3 protein gamma (YWHAG) ELISA Kit

14-3-3 protein gamma (YWHAG)

14-3-3 protein gamma; 14-3-3 protein gamma; 14-3-3 protein gamma; KCIP-1; 14-3-3 gamma; protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma; Protein kinase C inhibitor protein 1; KCIP-1

YWHAG; YWHAG; PPP1R170; 14-3-3GAMMA; KCIP-1

1433G_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of YWHAG. No significant cross-reactivity or interference between YWHAG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YWHAG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This YWHAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat YWHAG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: YWHAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-YWHAG antibody and an YWHAG-HRP conjugate. The assay sample and buffer are incubated together with YWHAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAG concentration since YWHAG from samples and YWHAG-HRP conjugate compete for the anti-YWHAG antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAG from the sample, fewer sites are left to bind YWHAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAG concentration in each sample is interpolated from this standard curve.

21464101

NP_036611.2

NM_012479.3

28,303 Da

Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Apoptosis Pathway (105648); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Centrosome Maturation Pathway (105807)

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; cytosol. Molecular Function: protein domain specific binding; insulin-like growth factor receptor binding; protein kinase C inhibitor activity; protein binding; protein kinase C binding; receptor tyrosine kinase binding. Biological Process: cellular response to insulin stimulus; apoptosis; regulation of neuron differentiation; organelle organization and biogenesis; regulation of synaptic plasticity; regulation of signal transduction; negative regulation of protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting

48-Strip-Wells

470 USD

96-Strip-Wells

675