Mouse Bone Morphogenetic Protein 1 ELISA Kit | MyBioSource MBS720542 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Bone Morphogenetic Protein 1 ELISA Kit

catalog :

MBS720542

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720542

products type :

ELISA Kit

products full name :

Mouse Bone Morphogenetic Protein 1 ELISA Kit

products short name :

Bone Morphogenetic Protein 1

other names :

bone morphogenetic protein 1 isoform 3; Bone morphogenetic protein 1; bone morphogenetic protein 1; mammalian tolloid protein; procollagen C-proteinase 3; procollagen C-endopeptidase; bone morphogenetic protein 1; Mammalian tolloid protein; mTld; Procollagen C-proteinase; PCP

products gene name :

BMP1

other gene names :

BMP1; BMP1; PCP; TLD; OI13; PCP2; PCOLC; PCOLC; BMP-1; mTld; PCP

uniprot entry name :

BMP1_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of BMP-1. No significant cross-reactivity or interference between BMP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BMP-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This BMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BMP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

. Principle of the assay: BMP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP-1 antibody and an BMP-1-HRP conjugate. The assay sample and buffer are incubated together with BMP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP-1 concentration since BMP-1 from samples and BMP-1-HRP conjugate compete for the anti-BMP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP-1 from the sample, fewer sites are left to bind BMP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5453579

ncbi acc num :

NP_006120.1

ncbi gb acc num :

NM_006129.4

ncbi mol weight :

92,655 Da

ncbi pathways :

Adipogenesis Pathway (198832); Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Cardiac Progenitor Differentiation Pathway (712094); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); HDL-mediated Lipid Transport Pathway (106158)

ncbi summary :

This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]

uniprot summary :

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Cytokine; EC 3.4.24.19. Chromosomal Location of Human Ortholog: 8p21.3. Cellular Component: Golgi apparatus; extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: peptidase activity; growth factor activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; cytokine activity; calcium ion binding. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; ossification; multicellular organismal development; lipoprotein metabolic process; cell differentiation; proteolysis; skeletal development; cartilage condensation. Disease: Osteogenesis Imperfecta, Type Xiii

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage