ELISA/assay

Rat B cell differentiation factor ELISA Kit

MBS720531

96 Strip Wells

675 USD

ELISA Kit

Rat B cell differentiation factor ELISA Kit

B cell differentiation factor

B cell differentiation factor I; Interleukin-5; interleukin-5; T-cell replacing factor; B-cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF

BCDE

IL5; IL5; EDF; TRF; IL-5; IL-5; TRF

IL5_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of BCDF. No significant cross-reactivity or interference between BCDF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCDF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This BCDF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat BCDF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Cell Biology

Principle of the assay: BCDF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCDF antibody and an BCDF-HRP conjugate. The assay sample and buffer are incubated together with BCDF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCDF concentration since BCDF from samples and BCDF-HRP conjugate compete for the anti-BCDF antibody binding site. Since the number of sites is limited, as more sites are occupied by BCDF from the sample, fewer sites are left to bind BCDF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCDF concentration in each sample is interpolated from this standard curve.

29393

CAA31211.1

P05113

15,238 Da

Allograft Rejection Pathway 920963!!Allograft Rejection Pathway 83123!!Allograft Rejection Pathway 535!!Asthma Pathway 83120!!Asthma Pathway 532!!Autoimmune Thyroid Disease Pathway 83121!!Autoimmune Thyroid Disease Pathway 533!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

96 Strip Wells

675 USD