ELISA/assay

Mouse A Kinase Anchor Protein 1 ELISA Kit

MBS720515

96 Strip Wells

675 USD

ELISA Kit

Mouse A Kinase Anchor Protein 1 ELISA Kit

A Kinase Anchor Protein 1

A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 1, mitochondrial; AKAP 149; D-AKAP-1; protein kinase A1; tudor domain containing 17; A-kinase anchor protein 149 kDa; protein kinase A anchoring protein 1; spermatid A-kinase anchor protein 84; protein phosphatase 1, regulatory subunit 43; dual-specificity A-kinase anchoring protein 1; A kinase (PRKA) anchor protein 1; A-kinase anchor protein 149 kDa; AKAP 149; Dual specificity A-kinase-anchoring protein 1; D-AKAP-1; Protein kinase A-anchoring protein 1; PRKA1; Spermatid A-kinase anchor protein 84; S-AKAP84

AKAP1

AKAP1; AKAP1; AKAP; PRKA1; AKAP84; TDRD17; AKAP121; AKAP149; D-AKAP1; PPP1R43; SAKAP84; AKAP149; PRKA1; AKAP 149; D-AKAP-1; PRKA1; S-AKAP84

AKAP1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of AKAP1. No significant cross-reactivity or interference between AKAP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKAP1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This AKAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AKAP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Signal Transduction

Principle of the assay: AKAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKAP1 antibody and an AKAP1-HRP conjugate. The assay sample and buffer are incubated together with AKAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKAP1 concentration since AKAP1 from samples and AKAP1-HRP conjugate compete for the anti-AKAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKAP1 from the sample, fewer sites are left to bind AKAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKAP1 concentration in each sample is interpolated from this standard curve.

339275976

NP_001229832.1

NM_001242903.1

62,921 Da

Factors Involved In Megakaryocyte Development And Platelet Production Pathway 187196!!G Protein Signaling Pathways 198849!!Hemostasis Pathway 106028

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD