ELISA/assay

Rat P 38 Mitogen-activated Protein Kinase ELISA Kit

MBS720509

48-Strip-Wells

470 USD

ELISA Kit

Rat P 38 Mitogen-activated Protein Kinase ELISA Kit

P 38 Mitogen-activated Protein Kinase

p38 map kinase; p38 map kinase; p38 map kinase<

P38MAPK

P38mapk

Rat

This assay has high sensitivity and excellent specificity for detection of P38MAPK. No significant cross-reactivity or interference between P38MAPK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between P38MAPK and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL. Assay Type: Competitive

Intended Uses: This P38MAPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat P38MAPK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: P38MAPK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P38MAPK antibody and an P38MAPK-HRP conjugate. The assay sample and buffer are incubated together with P38MAPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P38MAPK concentration since P38MAPK from samples and P38MAPK-HRP conjugate compete for the anti-P38MAPK antibody binding site. Since the number of sites is limited, as more sites are occupied by P38MAPK from the sample, fewer sites are left to bind P38MAPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P38MAPK concentration in each sample is interpolated from this standard curve.

218749850

NP_001136337.1

NM_001142865.1

FoxO Signaling Pathway (921443)

48-Strip-Wells

470 USD

96-Strip-Wells

675