ELISA/assay

Rabbit Claudin-18 (CLDN18) ELISA Kit

MBS7204960

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Claudin-18 (CLDN18) ELISA Kit

Claudin-18 (CLDN18)

claudin-18 isoform 2; Claudin-18; claudin-18; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J; claudin 18

CLDN18; CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572

CLD18_HUMAN

Rabbit

This assay has high sensitivity and excellent specificity for detection of CLDN-18. No significant cross-reactivity or interference between CLDN-18 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLDN-18 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL

Signal Transduction

Intended Uses: This CLDN-18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CLDN-18. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CLDN-18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLDN-18 antibody and an CLDN-18-HRP conjugate. The assay sample and buffer are incubated together with CLDN-18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN-18 concentration since CLDN-18 from samples and CLDN-18-HRP conjugate compete for the anti-CLDN-18 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN-18 from the sample, fewer sites are left to bind CLDN-18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN-18 concentration in each sample is interpolated from this standard curve.

50345279

NP_001002026.1

NM_001002026.2

27,720 Da

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Tight Junction Pathway (83071)

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010]

Claudin-18: a multi-pass membrane protein with calcium-independent cell-adhesion activity that plays a major role in the formation of tight junctions. Belongs to the claudin family. Two isoforms of the human protein are produced by alternative splicing. Isoform 2 is a specific cell lineage marker. Its expression in normal tissues is restricted to differentiated epithelial cells of the gastric mucosa; it is absent from the gastric stem cells. Isoform 2 is expressed in a significant proportion of primary gastric cancers and metastases, as well as in subtypes of pancreatic, esophageal, ovarian, and lung tumors. Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 3q22.3. Cellular Component: tight junction; integral to membrane; plasma membrane. Molecular Function: identical protein binding; structural molecule activity. Biological Process: intercellular junction assembly and maintenance; calcium-independent cell-cell adhesion

48-Strip-Wells

470 USD

96-Strip-Wells

675