ELISA/assay

Rat Gamma Glutamyltransferase 1 ELISA Kit

MBS720493

48-Strip-Wells

470 USD

ELISA Kit

Rat Gamma Glutamyltransferase 1 ELISA Kit

Gamma Glutamyltransferase 1

GGT1; Gamma-glutamyltranspeptidase 1; gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase; gamma-glutamyltransferase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain

gGT1

GGT1; GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732; GGT; GGT 1

GGT1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of GGT-1. No significant cross-reactivity or interference between GGT-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GGT-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 ng/mL.

Intended Uses: This GGT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GGT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: GGT-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GGT-1. Standards or samples are then added to the microtiter plate wells and GGT-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GGT-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GGT-1 are added to each well to "sandwich" the GGT-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GGT-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GGT-1 concentration in each sample is interpolated from this standard curve.

47678519

CAG30380.1

P19440

24,080 Da

Aflatoxin Activation And Detoxification Pathway (1016173); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (105698); Cyanoamino Acid Metabolism Pathway (82970); Cyanoamino Acid Metabolism Pathway (339); Eicosanoid Synthesis Pathway (198888); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973)

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: EC 3.4.19.14; Other Amino Acids Metabolism - selenoamino acid; Transferase; Other Amino Acids Metabolism - taurine and hypotaurine; EC 2.3.2.2; Membrane protein, integral; Other Amino Acids Metabolism - glutathione; EC 3.4.19.13; Other Amino Acids Metabolism - cyanoamino acid; Lipid Metabolism - arachidonic acid. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: anchored to external side of plasma membrane; extracellular space; integral to membrane; plasma membrane. Molecular Function: protein binding; gamma-glutamyltransferase activity. Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; glutathione catabolic process; regulation of inflammatory response; glutathione biosynthetic process; arachidonic acid metabolic process; zymogen activation; spermatogenesis. Disease: Glutathionuria

48-Strip-Wells

470 USD

96-Strip-Wells

675