Guinea pig Interleukin 33 ELISA Kit | MyBioSource MBS720482 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig Interleukin 33 ELISA Kit

catalog :

MBS720482

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720482

products type :

ELISA Kit

products full name :

Guinea pig Interleukin 33 ELISA Kit

products short name :

Interleukin 33

other names :

interleukin-33 isoform 3; Interleukin-33; interleukin-33; IL-33; IL-1F11; OTTHUMP00000021041; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules; interleukin 33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules

products gene name :

IL-33

other gene names :

IL33; IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; DKFZp586H0523; RP11-575C20.2; C9orf26; IL1F11; NFHEV

uniprot entry name :

IL33_HUMAN

reactivity :

Guinea Pig

specificity :

This assay has high sensitivity and excellent specificity for detection of IL-33/ST2. No significant cross-reactivity or interference between IL-33/ST2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-33/ST2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

products categories :

Guinea Pig ELISA Kit

products description :

Intended Uses: This IL-33/ST2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea IL-33/ST2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay IL-33/ST2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-33/ST2 antibody and an IL-33/ST2-HRP conjugate. The assay sample and buffer are incubated together with IL-33/ST2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-33/ST2 concentration since IL-33/ST2 from samples and IL-33/ST2-HRP conjugate compete for the anti-IL-33/ST2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-33/ST2 from the sample, fewer sites are left to bind IL-33/ST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-33/ST2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

314122337

ncbi acc num :

NP_001186570.1

ncbi gb acc num :

NM_001199641.1

uniprot acc num :

O95760

ncbi mol weight :

30,759 Da

ncbi pathways :

Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Influenza A Pathway (217173); Influenza A Pathway (217150)

ncbi summary :

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM]

uniprot summary :

Function: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines. Ref.3. Subcellular location: Secreted Ref.1 Ref.3. Tissue specificity: Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. Post-translational modification: Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Sequence similarities: Belongs to the IL-1 family. Highly divergent. Caution: Was originally (Ref.1) thought to one of the key factors that controls the specialized post-capillary high endothelial venules (HEV) phenotype found in organized secondary lymphoid tissue and to be nuclear localized.

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage