ELISA/assay

Porcine Telomeric repeat-binding factor 2 (TERF2) ELISA Kit

MBS7204607

48-Strip-Wells

470 USD

ELISA Kit

Porcine Telomeric repeat-binding factor 2 (TERF2) ELISA Kit

Telomeric repeat-binding factor 2 (TERF2)

telomeric repeat-binding factor 2; Telomeric repeat-binding factor 2; telomeric repeat-binding factor 2; telomeric DNA-binding protein; TTAGGG repeat-binding factor 2; telomeric repeat binding protein 2; telomeric repeat binding factor 2; TTAGGG repeat-binding factor 2; Telomeric DNA-binding protein

TERF2

TERF2; TERF2; TRF2; TRBF2; TRBF2; TRF2

TERF2_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of TERF2. No significant cross-reactivity or interference between TERF2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TERF2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This TERF2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine TERF2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

Principle of the assay: TERF2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TERF2 antibody and an TERF2-HRP conjugate. The assay sample and buffer are incubated together with TERF2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TERF2 concentration since TERF2 from samples and TERF2-HRP conjugate compete for the anti-TERF2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TERF2 from the sample, fewer sites are left to bind TERF2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TERF2 concentration in each sample is interpolated from this standard curve.

429535832

NP_005643.2

NM_005652.4

32,298 Da

Cell Cycle Pathway (530733); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromosome Maintenance Pathway (161048); DNA Damage/Telomere Stress Induced Senescence Pathway (905994); Meiosis Pathway (477133); Meiotic Synapsis Pathway (477134); Packaging Of Telomere Ends Pathway (106548); Regulation Of Telomerase Pathway (137987); Shelterin Complex Pathway (470348)

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq, Jul 2008]

TRF2: Binds the telomeric double-stranded 5 -TTAGGG-3 repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5 -TTAGGG-3 repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3 single- stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology- directed repair (HDR), which can affect telomere length. Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct. Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; DNA repair, damage. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: male germ cell nucleus; nucleoplasm; Golgi apparatus; chromosome, telomeric region; nuclear telomere cap complex; Mre11 complex; nuclear chromosome, telomeric region; cytoplasm; nucleus. Molecular Function: protein C-terminus binding; protein binding; protein homodimerization activity; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding. Biological Process: negative regulation of telomere maintenance; telomere capping; in utero embryonic development; negative regulation of telomere maintenance via semi-conservative replication; positive regulation of nitric-oxide synthase activity; telomeric loop formation; positive regulation of telomere maintenance; telomere maintenance via telomerase; age-dependent telomere shortening; cell cycle; telomere maintenance; protection from non-homologous end joining at telomere

48-Strip-Wells

470 USD

96-Strip-Wells

675