ELISA/assay

Mouse Fatty Acid Binding Protein 4, Adipocyte ELISA Kit

MBS720427

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Mouse Fatty Acid Binding Protein 4, Adipocyte ELISA Kit

Fatty Acid Binding Protein 4, Adipocyte

fatty acid binding protein 4, adipocyte; Fatty acid-binding protein, adipocyte; fatty acid-binding protein, adipocyte; fatty acid-binding protein 4; adipocyte lipid-binding protein; epididymis secretory protein Li 104; adipocyte-type fatty acid-binding protein; fatty acid binding protein 4, adipocyte; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4

FABP4

FABP4; FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104; ALBP; A-FABP; AFABP

FABP4_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of FABP4. No significant cross-reactivity or interference between FABP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FABP4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive or Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This FABP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FABP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: FABP4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FABP4. Standards or samples are then added to the microtiter plate wells and FABP4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FABP4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FABP4 are added to each well to “sandwich” the FABP4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FABP4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP4 concentration in each sample is interpolated from this standard curve.

119607498

EAW87092.1

14,719 Da

Developmental Biology Pathway (477129); Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway (106121); Lipid Digestion, Mobilization, And Transport Pathway (106111); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450); Transcriptional Regulation Of White Adipocyte Differentiation Pathway (205243)

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family. Protein type: Lipid-binding. Chromosomal Location of Human Ortholog: 8q21. Cellular Component: cytoplasm; lipid particle; nucleus. Molecular Function: transporter activity; fatty acid binding. Biological Process: cholesterol homeostasis; transport; white fat cell differentiation; triacylglycerol catabolic process; brown fat cell differentiation; cytokine production; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675