Human A Kinase Anchor Protein 1 ELISA Kit | MyBioSource MBS720412 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human A Kinase Anchor Protein 1 ELISA Kit

catalog :

MBS720412

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720412

products type :

ELISA Kit

products full name :

Human A Kinase Anchor Protein 1 ELISA Kit

products short name :

A Kinase Anchor Protein 1

other names :

A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 1, mitochondrial; AKAP 149; D-AKAP-1; protein kinase A1; tudor domain containing 17; A-kinase anchor protein 149 kDa; protein kinase A anchoring protein 1; spermatid A-kinase anchor protein 84; protein phosphatase 1, regulatory subunit 43; dual-specificity A-kinase anchoring protein 1; A kinase (PRKA) anchor protein 1; A-kinase anchor protein 149 kDa; AKAP 149; Dual specificity A-kinase-anchoring protein 1; D-AKAP-1; Protein kinase A-anchoring protein 1; PRKA1; Spermatid A-kinase anchor protein 84; S-AKAP84

products gene name :

AKAP1

other gene names :

AKAP1; AKAP1; AKAP; PRKA1; AKAP84; TDRD17; AKAP121; AKAP149; D-AKAP1; PPP1R43; SAKAP84; AKAP149; PRKA1; AKAP 149; D-AKAP-1; PRKA1; S-AKAP84

uniprot entry name :

AKAP1_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Signal Transduction

products description :

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE: This BMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BMP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY: BMP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP-1 antibody and an BMP-1-HRP conjugate. The assay sample and buffer are incubated together with BMP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP-1 concentration since BMP-1 from samples and BMP-1-HRP conjugate compete for the anti-BMP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP-1 from the sample, fewer sites are left to bind BMP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

339275976

ncbi acc num :

NP_001229832.1

ncbi gb acc num :

NM_001242903.1

ncbi mol weight :

62,921 Da

ncbi pathways :

Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); G Protein Signaling Pathways (198849); Hemostasis Pathway (106028)

ncbi summary :

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008]

uniprot summary :

AKAP1: Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma. 2 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding; Adaptor/scaffold; Membrane protein, integral; Mitochondrial. Chromosomal Location of Human Ortholog: 17q22. Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; integral to membrane; cytosol. Molecular Function: protein binding; RNA binding. Biological Process: blood coagulation

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage