ELISA/assay

Human Glioma pathogenesis related protein 1 ELISA Kit

MBS7202963

48-Strip-Wells

470 USD

ELISA Kit

Human Glioma pathogenesis related protein 1 ELISA Kit

Glioma pathogenesis related protein 1

glioma pathogenesis-related protein 1; Glioma pathogenesis-related protein 1; glioma pathogenesis-related protein 1; gliPR 1; protein RTVP-1; GLI pathogenesis-related 1 (glioma); testes-specific vespid and pathogenesis protein 1; related to testis-specific, vespid, and pathogenesis proteins 1; GLI pathogenesis-related 1; Protein RTVP-1

GLIPR1

GLIPR1; GLIPR1; GLIPR; RTVP1; CRISP7; GLIPR; RTVP1; GliPR 1

GLIP1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of GPR-1. No significant cross-reactivity or interference between GPR-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPR-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GPR-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GPR-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: GPR-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPR-1 antibody and an GPR-1-HRP conjugate. The assay sample and buffer are incubated together with GPR-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPR-1 concentration since GPR-1 from samples and GPR-1-HRP conjugate compete for the anti-GPR-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPR-1 from the sample, fewer sites are left to bind GPR-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPR-1 concentration in each sample is interpolated from this standard curve.

110825980

NP_006842.2

NM_006851.2

26,919 Da

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544)

This gene encodes a protein with similarity to both the pathogenesis-related protein (PR) superfamily and the cysteine-rich secretory protein (CRISP) family. Increased expression of this gene is associated with myelomocytic differentiation in macrophage and decreased expression of this gene through gene methylation is associated with prostate cancer. The protein has proapoptotic activities in prostate and bladder cancer cells. This gene is a member of a cluster on chromosome 12 containing two other similar genes. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

GLIPR1: a protein with similarity to both the pathogenesis-related protein (PR) superfamily and the cysteine-rich secretory protein (CRISP) family. Increased expression of this gene is associated with myelomocytic differentiation in macrophage and decreased expression of this gene through gene methylation is associated with prostate cancer. The protein has proapoptotic activities in prostate and bladder cancer cells. This gene is a member of a cluster on chromosome 12 containing two other similar genes. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q21.2. Cellular Component: membrane; integral to membrane; extracellular region; plasma membrane. Biological Process: cellular lipid metabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675