catalog number :
MBS720249
products type :
ELISA Kit
products full name :
Rat Lactic Acid ELISA Kit
products short name :
Lactic Acid
other names :
Lactic acid dehydrogenase; Lactic acid dehydrogenase; Lactic acid dehydrogenase
uniprot entry name :
G8SD27_ACTS5
specificity :
This assay has high sensitivity and excellent specificity for detection of LA. No significant cross-reactivity or interference between LA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 mmol/L.
other info2 :
Intended Uses: This LA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat LA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: LA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LA. Standards or samples are then added to the microtiter plate wells and LA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LA are added to each well to "sandwich" the LA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AEV84037.1
ncbi mol weight :
104,585 Da