ELISA/assay

Rabbit Acetylcholinesterase ELISA Kit

MBS720206

96 Strip Wells

675 USD

ELISA Kit

Rabbit Acetylcholinesterase ELISA Kit

Acetylcholinesterase

acetylcholinesterase; Acetylcholinesterase; acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase; acetylcholinesterase (Yt blood group)

AChE

ACHE; ACHE; YT; ACEE; ARACHE; N-ACHE; AChE

ACES_HUMAN

Rabbit

Store all reagents at 2-8 degree C.

Neurobiology

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This ACHE ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ACHE in the sample, this ACHE ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ACHE concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ACHE utilizes a multiclonal anti-ACHE antibody and an ACHE-HRP conjugate. The assay sample and buffer are incubated together with ACHE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACHE concentration since ACHE from samples and ACHE-HRP conjugate compete for the anti-ACHE antibody binding site. Since the number of sites is limited, as more sites are occupied by ACHE from the sample, fewer sites are left to bind ACHE-HRP conjugate. Standards of known ACHE concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of ACHE. The ACHE concentration in each sample is interpolated from this standard curve.

177975

AAA68151.1

58,352 Da

ATF-2 Transcription Factor Network Pathway 138006!!Acetylcholine Synthesis Pathway 198820!!Biogenic Amine Synthesis Pathway 198793!!Cholinergic Synapse Pathway 217716!!Glycerophospholipid Biosynthesis Pathway 645313!!Glycerophospholipid Metabolism Pathway 82989!!Glycerophospholipid Metabolism Pathway 364!!Integrated Pancreatic Cancer Pathway 711360!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD