catalog number :
MBS7201757
products type :
ELISA Kit
products full name :
Monkey Lipocalin-Type Prostaglandin D Synthase ELISA Kit
products short name :
Lipocalin-Type Prostaglandin D Synthase
other names :
lipocalin-type prostaglandin D synthase; Chondrogenesis associated lipocalin; prostaglandin-H2 D-isomerase; prostaglandin D2 synthase 21kDa (brain); Chondrogenesis associated lipocalin; Lipocalin-type prostaglandin D synthase
products gene name :
L-PGDS
other gene names :
PTGDS; L-PGDS; PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
uniprot entry name :
Q8QFM7_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of LPGD2S. No significant cross-reactivity or interference between LPGD2S and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LPGD2S and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This LPGD2S ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey LPGD2S. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: LPGD2S ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LPGD2S antibody and an LPGD2S-HRP conjugate. The assay sample and buffer are incubated together with LPGD2S-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LPGD2S concentration since LPGD2S from samples and LPGD2S-HRP conjugate compete for the anti-LPGD2S antibody binding site. Since the number of sites is limited, as more sites are occupied by LPGD2S from the sample, fewer sites are left to bind LPGD2S-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPGD2S concentration in each sample is interpolated from this standard curve.
ncbi acc num :
BAC06839.1
ncbi mol weight :
20,844 Da
ncbi pathways :
Arachidonic Acid Metabolism Pathway (83902); Arachidonic Acid Metabolism Pathway (366); Metabolic Pathways (132966)