ELISA/assay

Monkey cathepsin B (CTSB) ELISA Kit

MBS7201749

48-Strip-Wells

470 USD

ELISA Kit

Monkey cathepsin B (CTSB) ELISA Kit

cathepsin B (CTSB)

cathepsin B; Cathepsin B; cathepsin B; BCSB; cathepsin B<; BCSBCleaved into the following 2 chains:Cathepsin B light chain; Cathepsin B heavy chain

CTSB

CTSB; CTSB

CATB_BOVIN

Monkey

This assay has high sensitivity and excellent specificity for detection of CTSB. No significant cross-reactivity or interference between CTSB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTSB and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CTSB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CTSB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: CTSB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTSB antibody and an CTSB-HRP conjugate. The assay sample and buffer are incubated together with CTSB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTSB concentration since CTSB from samples and CTSB-HRP conjugate compete for the anti-CTSB antibody binding site. Since the number of sites is limited, as more sites are occupied by CTSB from the sample, fewer sites are left to bind CTSB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTSB concentration in each sample is interpolated from this standard curve.

27806671

NP_776456.1

NM_174031.2

36,661 Da

Antigen Processing And Presentation Pathway (84313); Antigen Processing And Presentation Pathway (485); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1008841); Collagen Degradation Pathway (1008853); Collagen Formation Pathway (1008839); Degradation Of The Extracellular Matrix Pathway (1008851); Extracellular Matrix Organization Pathway (1008838); Immune System Pathway (1009079); Innate Immune System Pathway (1009108); Lysosome Pathway (98762)

48-Strip-Wells

470 USD

96-Strip-Wells

675