ELISA/assay

Human Dihydrofolate reductase (DHFR) ELISA Kit

MBS7201709

48-Strip-Wells

470 USD

ELISA Kit

Human Dihydrofolate reductase (DHFR) ELISA Kit

Dihydrofolate reductase (DHFR)

dihydrofolate reductase; Dihydrofolate reductase; dihydrofolate reductase; dihydrofolate reductase

DHFR

Dhfr; Dhfr; AA607882; AI662710; AW555094; 8430436I03Rik

DYR_MOUSE

Human

This assay has high sensitivity and excellent specificity for detection of DHFR. No significant cross-reactivity or interference between DHFR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DHFR and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This DHFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DHFR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: DHFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DHFR antibody and an DHFR-HRP conjugate. The assay sample and buffer are incubated together with DHFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DHFR concentration since DHFR from samples and DHFR-HRP conjugate compete for the anti-DHFR antibody binding site. Since the number of sites is limited, as more sites are occupied by DHFR from the sample, fewer sites are left to bind DHFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHFR concentration in each sample is interpolated from this standard curve.

7106289

NP_034179.1

NM_010049.3

21,606 Da

Cell Cycle Pathway (971095); Cell Cycle, Mitotic Pathway (971092); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (970806); Defective BTD Causes Biotidinase Deficiency Pathway (971178); Defective CD320 Causes Methylmalonic Aciduria Pathway (970626); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (970808); Defective GIF Causes Intrinsic Factor Deficiency Pathway (970697); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (970511); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (970698); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (970570)

DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family. Protein type: EC 1.5.1.3; Cofactor and Vitamin Metabolism - one carbon pool by folate; Cofactor and Vitamin Metabolism - folate biosynthesis; Oxidoreductase. Molecular Function: mRNA binding; dihydrofolic acid binding; RNA binding; dihydrofolate reductase activity; drug binding; oxidoreductase activity; NADP binding. Biological Process: tetrahydrofolate metabolic process; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; dihydrofolate metabolic process; response to methotrexate; one-carbon compound metabolic process; glycine biosynthetic process

48-Strip-Wells

470 USD

96-Strip-Wells

675