ELISA/assay

Mouse Chemokine C-X-C-Motif Receptor 4 ELISA Kit

MBS720170

96 Strip Wells

675 USD

ELISA Kit

Mouse Chemokine C-X-C-Motif Receptor 4 ELISA Kit

Chemokine C-X-C-Motif Receptor 4

CD1; CD1

CXCR4

LOC443442

Mouse

Store all reagents at 2-8 degree C.

Immunology

Intended Uses: This CD1 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCD1 in the sample, thisCD1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCD1 concentration. The concentration ofCD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This CD1 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCD1. Standards or samples are then added to the microtiter plate wells andCD1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCD1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCD1 are added to each well to "sandwich" theCD1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCD1 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

57164257

NP_001009425.1

NM_001009425.1

Hematopoietic Cell Lineage Pathway 1030802!!Hematopoietic Cell Lineage Pathway 489

96 Strip Wells

675 USD