ELISA/assay

Rat Mannose Binding Lectin C ELISA Kit

MBS720153

48-Strip-Wells

470 USD

ELISA Kit

Rat Mannose Binding Lectin C ELISA Kit

Mannose Binding Lectin C

mannose-binding protein C; Mannose-binding protein C; mannose-binding protein C; RARF/P28A; mannan-binding protein; mannose binding lectin (C); RA-reactive factor P28A subunit; mannose binding lectin, liver (C); mannose-binding lectin (protein C) 2; Mannan-binding protein; RA-reactive factor P28A subunit; RARF/P28A

MBL-C

Mbl2; Mbl2; MBL; L-MBP; MBL-C; MBP-C; MBP-C; RARF/P28A

MBL2_MOUSE

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.01 ng/mL.

Intended Uses: This MBL-C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MBL-C. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: MBL-C ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MBL-C. Standards or samples are then added to the microtiter plate wells and MBL-C if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MBL-C present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MBL-C are added to each well to "sandwich" the MBL-C immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MBL-C and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBL-C concentration in each sample is interpolated from this standard curve.

6754656

NP_034906.1

NM_010776.1

25,957 Da

Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1001237); Creation Of C4 And C2 Activators Pathway (1001239); Immune System Pathway (971216); Initial Triggering Of Complement Pathway (1001238); Innate Immune System Pathway (971212); Lectin Pathway Of Complement Activation (1001240); Phagosome Pathway (153914); Phagosome Pathway (153859)

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; collagen; extracellular region. Molecular Function: mannose binding; protein self-association; protease binding; galactose binding; calcium ion binding; carbohydrate binding; calcium-dependent protein binding; receptor binding. Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; immune system process; positive regulation of phagocytosis; innate immune response; complement activation, lectin pathway; negative regulation of viral reproduction; complement activation, classical pathway

48-Strip-Wells

470 USD

96-Strip-Wells

675