ELISA/assay

Guinea pig Apoprotein B100 ELISA Kit

MBS720138

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig Apoprotein B100 ELISA Kit

Apoprotein B100

apo-B100; Apolipoprotein B-100; apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen); apolipoprotein B

Apo-B100

APOB; APOB; FLDB; LDLCQ4; Apo B-100; Apo B-48

APOB_HUMAN

Guinea Pig

This assay has high sensitivity and excellent specificity for detection of Apo-B100. No significant cross-reactivity or interference between Apo-B100 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Apo-B100 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This Apo-B100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig Apo-B100. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Princple of the assay: Apo-B100 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Apo-B100 antibody and an Apo-B100-HRP conjugate. The assay sample and buffer are incubated together with Apo-B100-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Apo-B100 concentration since Apo-B100 from samples and Apo-B100-HRP conjugate compete for the anti-Apo-B100 antibody binding site. Since the number of sites is limited, as more sites are occupied by Apo-B100 from the sample, fewer sites are left to bind Apo-B100-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo-B100 concentration in each sample is interpolated from this standard curve.

28780

CAA28420.1

P04114

515,605 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA1 Transcription Factor Network Pathway (137979); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (106028); LDL-mediated Lipid Transport Pathway (106159)

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. Protein type: Secreted; Secreted, signal peptide; Carrier. Chromosomal Location of Human Ortholog: 2p24-p23. Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; chylomicron; intracellular membrane-bound organelle; cell soma; endoplasmic reticulum lumen; cytoplasm; early endosome; extracellular region; plasma membrane; endosome membrane; cytosol; actin cytoskeleton. Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding. Biological Process: lipoprotein catabolic process; phototransduction, visible light; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; cholesterol metabolic process; nervous system development; receptor-mediated endocytosis; cholesterol transport; in utero embryonic development; regulation of cholesterol biosynthetic process; response to virus; cholesterol efflux; lipoprotein metabolic process; cholesterol homeostasis; sperm motility; fertilization; lipoprotein biosynthetic process; lipoprotein transport; artery morphogenesis; spermatogenesis; blood coagulation; triacylglycerol mobilization; leukocyte migration. Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1

48-Strip-Wells

470 USD

96-Strip-Wells

675