Rat Tissue inhibitors of metalloproteinase 2 ELISA Kit | MyBioSource MBS720098 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Tissue inhibitors of metalloproteinase 2 ELISA Kit

catalog :

MBS720098

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720098

products type :

ELISA Kit

products full name :

Rat Tissue inhibitors of metalloproteinase 2 ELISA Kit

products short name :

Tissue inhibitors of metalloproteinase 2

other names :

tissue inhibitor of metalloproteinase type 2; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2; TIMP metallopeptidase inhibitor 2; Tissue inhibitor of metalloproteinases 2; TIMP-2

products gene name :

TIMP-2

other gene names :

Timp2; Timp2; Timp-2; TIMP-2

uniprot entry name :

TIMP2_RAT

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of TIMP-2. No significant cross-reactivity or interference between TIMP-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TIMP-2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This TIMP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TIMP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cancer

products description :

. Principle of the assay: TIMP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TIMP-2 antibody and an TIMP-2-HRP conjugate. The assay sample and buffer are incubated together with TIMP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TIMP-2 concentration since TIMP-2 from samples and TIMP-2-HRP conjugate compete for the anti-TIMP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TIMP-2 from the sample, fewer sites are left to bind TIMP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP-2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

619233

ncbi acc num :

AAC60687.1

ncbi mol weight :

24,356 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1009937); Degradation Of The Extracellular Matrix Pathway (1009936); Extracellular Matrix Organization Pathway (1009923); Matrix Metalloproteinases Pathway (198492)

ncbi summary :

acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006]

uniprot summary :

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell development/differentiation; Extracellular matrix; Inhibitor. Cellular Component: extracellular space; proteinaceous extracellular matrix; neuron projection; cell surface; growth cone; cell soma; basement membrane; nucleus. Molecular Function: integrin binding; metalloendopeptidase inhibitor activity; protease binding; metal ion binding; enzyme activator activity. Biological Process: negative regulation of proteolysis; regulation of Rap protein signal transduction; positive regulation of catalytic activity; response to drug; negative regulation of metalloenzyme activity; central nervous system development; regulation of neuron differentiation; regulation of cAMP metabolic process; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; spermatogenesis; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage