ELISA/assay

Guinea pig a-L-iduronidase ELISA Kit

MBS720087

96 Strip Wells

675 USD

ELISA Kit

Guinea pig a-L-iduronidase ELISA Kit

a-L-iduronidase

IDUA protein, partial; Alpha-L-iduronidase; alpha-L-iduronidase; iduronidase, alpha-L-

IDUA

IDUA; IDUA; IDA; MPS1

IDUA_HUMAN

Guinea Pig

This assay has high sensitivity and excellent specificity for detection of IDUA. No significant cross-reactivity or interference between IDUA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IDUA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This IDUA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig IDUA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Signal Transduction

Principle of the assay: IDUA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IDUA antibody and an IDUA-HRP conjugate. The assay sample and buffer are incubated together with IDUA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IDUA concentration since IDUA from samples and IDUA-HRP conjugate compete for the anti-IDUA antibody binding site. Since the number of sites is limited, as more sites are occupied by IDUA from the sample, fewer sites are left to bind IDUA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IDUA concentration in each sample is interpolated from this standard curve.

62089474

AAH29959.1

72,670 Da

CS/DS Degradation Pathway 645311!!Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway 645308!!Dermatan Sulfate Degradation Pathway 413375!!Dermatan Sulfate Degradation Pathway 468240!!Disease Pathway 530764!!Glycogen Storage Diseases Pathway 980468!!Glycosaminoglycan Degradation Pathway 82981!!Glycosaminoglycan Degradation Pathway 355!!Glycosaminoglycan Metabolism Pathway 645297!!HS-GAG Degradation Pathway 645307

This gene encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans. Mutations in this gene that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I). [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD