Guinea pig Calcineurin Binding Protein 1 ELISA Kit | MyBioSource MBS720072 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig Calcineurin Binding Protein 1 ELISA Kit

catalog :

MBS720072

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720072

products type :

ELISA Kit

products full name :

Guinea pig Calcineurin Binding Protein 1 ELISA Kit

products short name :

Calcineurin Binding Protein 1

other names :

calcineurin-binding protein cabin-1 isoform b; Calcineurin-binding protein cabin-1; calcineurin-binding protein cabin-1; calcineurin inhibitor; calcineurin binding protein cabin 1; calcineurin binding protein 1; Calcineurin inhibitor; CAIN

products gene name :

CABIN1

other gene names :

CABIN1; CABIN1; CAIN; PPP3IN; KIAA0330; CAIN

uniprot entry name :

CABIN_HUMAN

reactivity :

Guinea Pig

specificity :

This assay has high sensitivity and excellent specificity for detection of CABIN1. No significant cross-reactivity or interference between CABIN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CABIN1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This CABIN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig CABIN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: CABIN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CABIN1 antibody and an CABIN1-HRP conjugate. The assay sample and buffer are incubated together with CABIN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CABIN1 concentration since CABIN1 from samples and CABIN1-HRP conjugate compete for the anti-CABIN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CABIN1 from the sample, fewer sites are left to bind CABIN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CABIN1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

319738622

ncbi acc num :

NP_001188358.1

ncbi gb acc num :

NM_001201429.1

ncbi mol weight :

237,574 Da

ncbi pathways :

Calcium Signaling In The CD4+ TCR Pathway (137941); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); DNA Damage/Telomere Stress Induced Senescence Pathway (905994); Formation Of Senescence-Associated Heterochromatin Foci (SAHF) Pathway (905995); Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway (137953)

ncbi summary :

Calcineurin plays an important role in the T-cell receptor-mediated signal transduction pathway. The protein encoded by this gene binds specifically to the activated form of calcineurin and inhibits calcineurin-mediated signal transduction. The encoded protein is found in the nucleus and contains a leucine zipper domain as well as several PEST motifs, sequences which confer targeted degradation to those proteins which contain them. Alternative splicing results in multiple transcript variants encoding two different isoforms. [provided by RefSeq, Jan 2011]

uniprot summary :

CABIN1: an inhibitor of the calcium-dependent serine/threonine phosphatase, calcineurin. Inhibits calcineurin signaling in T cells, regulating T cell receptor (TCR) signaling, transcription, and apoptosis. Inhibition of activated calcineurin is dependent on both PKC and calcium signals. Represses myocyte enhancer factor 2 (MEF2)-mediated transcription by recruiting chromatin remodeling enzymes, and also negatively regulates the activity of the tumor suppressor p53. Is degraded in response to genotoxic stress, releasing its inhibition of p53, allowing p53 to elicit cellular stress responses. Involved in regulation of chromatin structure as part of the highly conserved HIRA/UBN1/CABIN1/ASF1A (HUCA) histone chaperone complex. Protein type: Protein phosphatase, regulatory subunit; Transcription, coactivator/corepressor; Chaperone. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: nucleoplasm; cytoplasm. Molecular Function: protein phosphatase inhibitor activity. Biological Process: cell surface receptor linked signal transduction; DNA replication-independent nucleosome assembly; negative regulation of catalytic activity; chromatin modification

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage