ELISA/assay

Human Annexin V ELISA Kit

MBS720067

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Annexin V ELISA Kit

Annexin V

annexin A5; annexin A5; annexin 5; annexin A5b

ANN V

anxa5b; anx; anx5; ANX V; anxa5; cb989; wu:fa98f06; wu:fj10f10

Human

This assay has high sensitivity and excellent specificity for detection of ANXA-5. No significant cross-reactivity or interference between ANXA-5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANXA-5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This ANXA-5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANXA-5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: ANXA-5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA-5 antibody and an ANXA-5-HRP conjugate. The assay sample and buffer are incubated together with ANXA-5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA-5 concentration since ANXA-5 from samples and ANXA-5-HRP conjugate compete for the anti-ANXA-5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA-5 from the sample, fewer sites are left to bind ANXA-5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA-5 concentration in each sample is interpolated from this standard curve.

40254661

NP_861422.2

NM_181757.2

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675