ELISA/assay

Mouse A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS2) ELISA Kit

MBS7200560

48-Strip-Wells

470 USD

ELISA Kit

Mouse A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS2) ELISA Kit

A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS2)

A disintegrin and metalloproteinase with thrombospondin motifs 2 isoform 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 2; A disintegrin and metalloproteinase with thrombospondin motifs 2; procollagen I N-proteinase; procollagen N-endopeptidase; procollagen I/II amino propeptide-processing enzyme; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2; ADAM metallopeptidase with thrombospondin type 1 motif, 2; Procollagen I N-proteinase; PC I-NP; Procollagen I/II amino propeptide-processing enzyme; Procollagen N-endopeptidase; pNPI

ADAMTS2

ADAMTS2; ADAMTS2; NPI; PNPI; PCINP; PCPNI; PCI-NP; PC I-NP; ADAM-TS2; ADAMTS-2; ADAMTS-3; PCINP; PCPNI; ADAM-TS 2; ADAM-TS2; ADAMTS-2; PC I-NP; pNPI

ATS2_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of ADAMTS2. No significant cross-reactivity or interference between ADAMTS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTS2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ADAMTS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ADAMTS2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: ADAMTS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAMTS2 antibody and an ADAMTS2-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS2 concentration since ADAMTS2 from samples and ADAMTS2-HRP conjugate compete for the anti-ADAMTS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS2 from the sample, fewer sites are left to bind ADAMTS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS2 concentration in each sample is interpolated from this standard curve.

110825974

NP_055059.2

NM_014244.4

61,756 Da

Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Extracellular Matrix Organization Pathway (576262); Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene excises the N-propeptide of type I, type II and type V procollagens. Mutations in this gene cause Ehlers-Danlos syndrome type VIIC, a recessively inherited connective-tissue disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

ADAMTS2: Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis. Defects in ADAMTS2 are the cause of Ehlers-Danlos syndrome type 7C (EDS7C). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7C is marked by extremely fragile tissues, hyperextensible skin and easy bruising. Facial skin contains numerous folds, as in the cutis laxa syndrome. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Extracellular matrix; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; EC 3.4.24.14. Chromosomal Location of Human Ortholog: 5qter. Cellular Component: proteinaceous extracellular matrix; extracellular region. Molecular Function: metallopeptidase activity; zinc ion binding; metalloendopeptidase activity. Biological Process: skin development; collagen catabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; spermatogenesis; protein processing; proteolysis; lung development. Disease: Ehlers-danlos Syndrome, Type Vii, Autosomal Recessive

48-Strip-Wells

470 USD

96-Strip-Wells

675