catalog number :
MBS720037
products type :
ELISA Kit
products full name :
Rabbit Thrombin-antithrombin complex ELISA Kit
products short name :
Thrombin-antithrombin complex
other names :
tat, partial; Protein Tat
uniprot entry name :
E2FMT9_9HIV1
specificity :
This assay has high sensitivity and excellent specificity for detection of TAT. No significant cross-reactivity or interference between TAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TAT and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This TAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit TAT. This ELISA kit for research use only, not for therapeutic or diagnostic applications
products categories :
Immunology
products description :
Principle of the assay: TAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TAT antibody and an TAT-HRP conjugate. The assay sample and buffer are incubated together with TAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color ismeasured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TAT concentration since TAT from samples and TAT-HRP conjugate compete for the anti-TAT antibody binding site. Since the number of sites is limited, as more sites are occupied by TAT from the sample, fewer sites are left to bind TAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAT concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ADK70247.1
ncbi mol weight :
8,343 Da