catalog number :
MBS720024
products type :
ELISA Kit
products full name :
Human Vitamin B6 ELISA Kit
products short name :
[Vitamin B6]
products gene name :
[VB6]
specificity :
This assay has high sensitivity and excellent specificity for detection of VB2. No significant cross-reactivity or interference between VB2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VB2 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Sandwich. Sensitivity: 1.0 ng/mL.
products description :
Intended Uses: This VB2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VB2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: VB2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VB2. Standards or samples are then added to the microtiter plate wells and VB2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VB2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VB2 are added to each well to "sandwich" the VB2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VB2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VB2 concentration in each sample is interpolated from this standard curve.
size4 :
10x96-Strip-Wells