ELISA/assay

Rat Pepsinogen C ELISA Kit

MBS720006

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Pepsinogen C ELISA Kit

Pepsinogen C

pepsinogen C; Gastricsin; Pepsinogen C

PGC

PGC

PEPC_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of PGC. No significant cross-reactivity or interference between PGC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This PGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PGC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: PGC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGC antibody and an PGC-HRP conjugate. The assay sample and buffer are incubated together with PGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGC concentration since PGC from samples and PGC-HRP conjugate compete for the anti-PGC antibody binding site. Since the number of sites is limited, as more sites are occupied by PGC from the sample, fewer sites are left to bind PGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGC concentration in each sample is interpolated from this standard curve.

387015

AAA60063.1

34,208 Da

PGC: Hydrolyzes a variety of proteins. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.23.3; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 6p21.1. Cellular Component: extracellular space. Molecular Function: aspartic-type endopeptidase activity. Biological Process: digestion; proteolysis

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675