ELISA/assay

Human Decorin ELISA Kit

MBS720005

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Decorin ELISA Kit

Decorin

decorin, partial; Decorin; Bone proteoglycan II; PG-S2; PG40

DCN

DCN; SLRR1B

PGS2_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Intended Uses: This DCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DCN. This ELISA kit for research use only, not for therapeutic or diagnostic applications. Intended Uses: This DCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DCN. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Principle of the Assay: DCN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DCN. Standards or samples are then added to the microtiter plate wells and DCN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DCN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DCN are added to each well to "sandwich" the DCN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DCN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCN concentration in each sample is interpolated from this standard curve.

609452

AAB60901.1

8,266 Da

DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q21.33. Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; proteinaceous extracellular matrix; Golgi lumen; extracellular region; collagen type VI. Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding. Biological Process: skeletal muscle development; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; wound healing; glycosaminoglycan metabolic process; response to lipopolysaccharide; pathogenesis; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; chondroitin sulfate catabolic process; carbohydrate metabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; placenta development; aging. Disease: Corneal Dystrophy, Congenital Stromal

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675