Rabbit anti-human alpha-2-macroglobulin polyclonal Antibody | MyBioSource MBS716966 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti-human alpha-2-macroglobulin polyclonal Antibody

catalog :

MBS716966

quantity :

0.05 mL

price :

375 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS716966

products type :

Antibody

products full name :

Rabbit anti-human alpha-2-macroglobulin polyclonal Antibody

products short name :

alpha-2-macroglobulin

products name syn :

alpha-2-macroglobulin; A2M; CPAMD5; DKFZp779B086; FWP007; S863-7

other names :

alpha-2-macroglobulin; Alpha-2-macroglobulin; alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

other gene names :

A2M; A2M; A2MD; CPAMD5; FWP007; S863-7; CPAMD5; Alpha-2-M

uniprot entry name :

A2MG_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human,Mouse,Rat; Other species are not tested. Please decide the specificity by homology.

sequence length :

1474

purity :

Antigen Affinity Purified

form :

Liquid

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Immunoprecipitation (IP)

app notes :

ELISA: Use at an assay dependent dilution. WB : 1:2000-1:20000 . IP : 1:200-1:1000 . IHC : 1:20-1:200 (Recommender dilutions). Positive WB detected in : Human blood tissue, HepG2 cells, human brain tissue, human serum tissue . Positive IP detected in: HepG2 cells Positive IHC detected in: Human hepatocirrhosis tissue, human ovary tissue, human stomach tissue

other info1 :

Immunogen: Human A2M. Conjugate: Non-conjugated

other info2 :

Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

products description :

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

ncbi gi num :

66932947

ncbi acc num :

NP_000005.2

ncbi gb acc num :

NM_000014.4

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); HDL-mediated Lipid Transport Pathway (106158); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Intrinsic Pathway (106059)

ncbi summary :

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

uniprot summary :

A2M: Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. Protein type: Inhibitor; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: extracellular region; cytosol. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding. Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway. Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency

size1 :

0.05 mL

price1 :

375 USD

size2 :

0.15 mL

price2 :

715

more info or order :

MyBioSource product webpage