Rabbit anti-human Wolf-Hirschhorn syndrome candidate 2 polyclonal Antibody | MyBioSource MBS711252 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti-human Wolf-Hirschhorn syndrome candidate 2 polyclonal Antibody

catalog :

MBS711252

quantity :

0.05 mL

price :

375 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS711252

products type :

Antibody

products full name :

Rabbit anti-human Wolf-Hirschhorn syndrome candidate 2 polyclonal Antibody

products short name :

Wolf-Hirschhorn syndrome candidate 2

products name syn :

Wolf-Hirschhorn syndrome candidate 2; WHSC2; FLJ10442; FLJ25112; NELF-A; NELFA; P/OKcl.15

other names :

Wolf-Hirschhorn syndrome candidate 2; Negative elongation factor A; negative elongation factor A; wolf-Hirschhorn syndrome candidate 2 protein; negative elongation factor complex member A; Wolf-Hirschhorn syndrome candidate 2 protein

other gene names :

NELFA; NELFA; WHSC2; NELF-A; P/OKcl.15; WHSC2; NELF-A

uniprot entry name :

NELFA_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human ,Mouse ;Other species are not tested. Please decide the specificity by homology.

sequence length :

528

purity :

Antigen Affinity Purified

form :

Liquid

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C. or -80 degree C. Avoid repeated freeze.

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

ELISA: Use at an assay dependent dilution. WB: 1:500-1:5000 . IF: 1:10-1:100 (Recommender dilutions) . Positive WB detected in: HeLa cells, Jurkat cells, mouse brain tissue, mouse liver tissue . Positive IF detected in: HeLa cells

other info1 :

Immunogen: Human NELF-A. Conjugate : Non-conjugated

other info2 :

Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

products description :

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.

ncbi gi num :

38114773

ncbi acc num :

AAH02764.2

ncbi mol weight :

28,416 Da

ncbi pathways :

Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway (106005); Disease Pathway (530764); Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway (161052); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); Formation Of RNA Pol II Elongation Complex Pathway (105950); Formation Of The Early Elongation Complex Pathway (105949); Formation Of The HIV-1 Early Elongation Complex Pathway (106002); Gene Expression Pathway (105937); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984)

ncbi summary :

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]

uniprot summary :

WHSC2: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. Belongs to the NELF-A family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 4p16.3. Cellular Component: nucleoplasm; cytoplasm. Molecular Function: protein binding; chromatin binding. Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; multicellular organismal development; RNA elongation from RNA polymerase II promoter; gene expression

size1 :

0.05 mL

price1 :

375 USD

size2 :

0.15 mL

price2 :

715

more info or order :

MyBioSource product webpage