product summary
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company name :
MyBioSource
product type :
antibody
product name :
Phospho-HIST1H1B (T154) Antibody
catalog :
MBS7106712
quantity :
0.05 mL
price :
165 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, enzyme immunoassay, chromatin immunoprecipitation
more info or order :
image
image 1 :
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: HIST1H1B antibody at 1.5ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 23 KDa. Observed band size: 32 KDa.
image 2 :
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1B (MBS7106712) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.
image 3 :
Immunofluorescence staining of HepG2 cells with MBS7106712 at 1:100, counter?stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488?congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
product information
catalog number :
MBS7106712
products type :
Antibody
products full name :
Phospho-HIST1H1B (T154) Antibody
products short name :
[HIST1H1B]
products name syn :
[Histone H1.5; Histone H1a; Histone H1b; Histone H1s-3; HIST1H1B; H1F5]
other names :
[histone H1.5; Histone H1.5; histone H1.5; histone cluster 1 H1 family member b; Histone H1a; Histone H1b; Histone H1s-3]
products gene name :
[HIST1H1B]
other gene names :
[HIST1H1B; HIST1H1B; H1; H1B; H1.5; H1F5; H1s-3; H1F5]
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human
sequence length :
226
purity :
Antigen Affinity Purified
form :
Liquid
storage stability :
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
tested application :
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
app notes :
Recommended dilution: WB: 1:200-1:2000, IF: 1:50-1:200
image1 heading :
Western Blot (WB)
image2 heading :
Chromatin Immunoprecipitation (ChIP)
image3 heading :
Immunofluorescence (IF)
other info1 :
Immunogen: Peptide sequence around site of phospho-Thr (154) derived from human Histone H1.5. Research Area: Epigenetics and Nuclear Signaling
other info2 :
Conjugation: Non-conjugated. Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
products categories :
Epigenetics and Nuclear Signaling
products description :
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
ncbi gi num :
4885381
ncbi acc num :
NP_005313.1
ncbi gb acc num :
NM_005322.2
uniprot acc num :
P16401
ncbi pathways :
Activation Of DNA Fragmentation Factor Pathway (105683); Apoptosis Pathway (105648); Apoptosis Induced DNA Fragmentation Pathway (105682); Apoptotic Execution Phase Pathway (105677)
ncbi summary :
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
uniprot summary :
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation ().
size1 :
0.05 mL
price1 :
165 USD
size2 :
0.1 mL
price2 :
250
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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