catalog number :
MBS707190
products type :
ELISA Kit
products full name :
Sheep adrenocorticotropic hormone, ACTH ELISA Kit
products short name :
[adrencocorticotropic hormone (ACTH)]
products name syn :
[Sheep adrenocorticotropic hormone (ACTH) ELISA kit; adrencocorticotropic hormone (ACTH)]
products gene name :
[ACTH]
specificity :
This assay has high sensitivity and excellent specificity for detection of sheep ACTH. No significant cross-reactivity or interference between sheep ACTH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 0.097 pg/ml -400 pg/ml. Sensitivity: The minimum detectable dose of sheep ACTH is typically less than 0.097 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest sheep ACTH retinoic acid concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for ACTH and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated ACTH and ACTH in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ACTH in the sample. The color development is stopped and the intensity of the color is measured.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells