ELISA/assay

Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC1A ELISA Kit

MBS707053

48-Strip-Wells

510 USD

ELISA Kit

Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC1A ELISA Kit

peroxisome proliferator-activated receptor gamma, coactivator 1 alpha

Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) ELISA kit; LEM6; PGC-1 (alpha); PGC-1v; PGC1; PGC1A; PPARGC1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; ligand effect modulator-6; peroxisome proliferative activated receptor; gamma; coactivat; peroxisome proliferator-activated receptor gamma; coactivator 1 alpha

peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; L-PGC-1alpha; PPARGC-1-alpha; ligand effect modulator-6; PPAR gamma coactivator variant form; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; Ligand effect modulator 6

PPARGC1A

PPARGC1A; PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha); LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha

PRGC1_HUMAN

Mouse

798

This assay has high sensitivity and excellent specificity for detection of mouse PPARGC1A. No significant cross-reactivity or interference between mouse PPARGC1A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of mouse PPARGC1A is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPARGC1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPARGC1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPARGC1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPARGC1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

7019499

NP_037393.1

NM_013261.3

Q9UBK2

91,027 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); ATF-2 Transcription Factor Network Pathway (138006); Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway (980487); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); Developmental Biology Pathway (477129)

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Function: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.4 PublicationsManual assertion based on experiment in:Ref.3

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800