catalog number :
MBS707028
products type :
ELISA Kit
products full name :
Human alphaII-spectrin breakdown products SBDP120, SBDP120 ELISA Kit
products short name :
alphaII-spectrin breakdown products SBDP120 (SBDP120)
products name syn :
Human alphaII-spectrin breakdown products SBDP120 (SBDP120) ELISA kit; alphaII-spectrin breakdown products SBDP120 (SBDP120)
products gene name :
SBDP120
specificity :
This assay has high sensitivity and excellent specificity for detection of human SBDP120. No significant cross-reactivity or interference between human SBDP120 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human SBDP120 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SBDP120 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SBDP120 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SBDP120 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SBDP120 bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells