catalog number :
MBS706891
products type :
ELISA Kit
products full name :
Dog Complement 4, C4 ELISA Kit
products short name :
Complement 4 (C4)
products name syn :
Dog Complement 4 (C4) ELISA kit; Complement 4 (C4)
other names :
complement 4; complement 4; complement C4; MHC-linked complement C4; complement 4
other gene names :
C4; C4-2
specificity :
This assay has high sensitivity and excellent specificity for detection of dog C4. No significant cross-reactivity or interference between dog C4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 31.25 ng/ml -2000 ng/ml. Sensitivity: The minimum detectable dose of dog C4 is typically less than 7.8 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with C4. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for C4. The competitive inhibition reaction is launched between with pre-coated C4 and C4 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of C4 in the samples. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001070701.1
ncbi gb acc num :
NM_001077233.1
size4 :
10x96-Strip-Wells