ELISA/assay

Rat Nuclear respiratory factor 2, NRF2 ELISA Kit

MBS706836

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Rat Nuclear respiratory factor 2, NRF2 ELISA Kit

[Nuclear respiratory factor 2 (NRF2)]

[Rat Nuclear respiratory factor 2 (NRF2) ELISA kit; Nuclear respiratory factor 2 (NRF2)]

[nuclear respiratory factor 1 isoform 1; Nuclear respiratory factor 1; nuclear respiratory factor 1; alpha palindromic-binding protein; nuclear respiratory factor 1; Alpha palindromic-binding protein; Alpha-pal]

[NRF2]

[NRF1; NRF1; ALPHA-PAL; NRF-1; Alpha-pal]

NRF1_HUMAN

Rat

503

This assay has high sensitivity and excellent specificity for detection of rat NRF2. No significant cross-reactivity or interference between rat NRF2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell lysates. Detection Range: 34.4 pg/ml-2200 pg/mL. Sensitivity: <8.6 pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

93141039

NP_001035199.1

NM_001040110.1

Q16656

55,674 Da

Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Mitochondrial Gene Expression Pathway (198757); Mitochondrial Biogenesis Pathway (980486); Organelle Biogenesis And Maintenance Pathway (980485); PPARA Activates Gene Expression Pathway (530749)

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Function: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145