ELISA/assay

Fish Aromatase, ARO ELISA Kit

MBS706813

96 Strip Wells

850 USD

ELISA Kit

Fish Aromatase, ARO ELISA Kit

cytochrome P450, family 19, subfamily A, polypeptide 1

Fish Aromatase (ARO) ELISA Kit; ARO; ARO1; CPV1; CYAR; CYP19; MGC104309; P-450AROM; OTTHUMP00000162543; aromatase; cytochrome P450; subfamily XIX (aromatization of androgens) ; estrogen synthetase; flavoprotein-linked monooxygenase; mic; cytochrome P450; family 19; subfamily A; polypeptide 1

aromatase; Aromatase; aromatase; CYPXIX; estrogen synthase; cytochrome P-450AROM; cytochrome P450 19A1; cytochrome P450, 19, aromatase; cytochrome P450, family 19, subfamily a, polypeptide 1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase

CYP19A1

Cyp19a1; Cyp19a1; Ar; ArKO; Int5; Cyp19; Int-5; p450arom; Arom; Cyp19

CP19A_MOUSE

Fish

503

This assay has high sensitivity and excellent specificity for detection of fish ARO. No significant cross-reactivity or interference between fish ARO and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of fish aromatase (ARO) concentrations in serum, plasma, tissue homogenates. Detection Range: 38.5 pg/ml-1000 pg/ml.

Sensitivity: The minimum detectable dose of fish ARO is typically less than 38.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest fish ARO concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for ARO and Horseradish Peroxidase (HRP) conjugated ARO. The competitive inhibition reaction is launched between with HRP labeled ARO and unlabeled ARO with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ARO in the sample. The color development is stopped and the intensity of the color is measured.

6681099

NP_031836.1

NM_007810.3

P28649

58,015 Da

Biological Oxidations Pathway 970688!!C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway 421781!!C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway 468268!!Cytochrome P450 Pathway 198390!!Cytochrome P450 - Arranged By Substrate Type Pathway 971395!!Endogenous Sterols Pathway 1001152!!Estrogen Biosynthesis Pathway 1001066!!Metabolic Pathways 132962!!Metabolism Pathway 971233!!Metabolism Of Lipids And Lipoproteins Pathway 970615

Function: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Catalytic activity: Testosterone + 3 O2 + 3 reduced flavoproteins = 17-beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins. Cofactor: Heme group

96 Strip Wells

850 USD