catalog number :
MBS706811
products type :
ELISA Kit
products full name :
Dog Insulin-like growth factor II, IGF2 ELISA Kit
products short name :
insulin-like growth factor 2 (somatomedin A)
products name syn :
Dog Insulin-like growth factor II (IGF2) ELISA kit; C11orf43; FLJ22066; FLJ44734; INSIGF; pp9974; OTTHUMP00000011018; OTTHUMP00000011157; insulin-like growth factor 2; insulin-like growth factor II; insulin-like growth factor type 2; putative insulin-like; insulin-like growth factor 2 (somatomedin A)
other names :
insulin-like growth factor II preproprotein; Insulin-like growth factor II; insulin-like growth factor II; insulin growth factor 2
products gene name :
IGF2
other gene names :
IGF2; IGF2; IGF-II; IGF-II
uniprot entry name :
IGF2_SHEEP
specificity :
This assay has high sensitivity and excellent specificity for detection of dog IGF2. No significant cross-reactivity or interference between dog IGF2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: The minimum detectable dose of dog IGF2 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001009311.1
ncbi gb acc num :
NM_001009311.1
ncbi mol weight :
19,616 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
size4 :
10x96-Strip-Wells