catalog number :
MBS706799
products type :
ELISA Kit
products full name :
Mouse anti-gastric parietal cell, GPC antibody ELISA Kit
products short name :
anti-gastric parietal cell (GPC) antibody
products name syn :
Mouse anti-gastric parietal cell (GPC) antibody ELISA kit; anti-gastric parietal cell (GPC) antibody
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse anti-GPC antibody. No significant cross-reactivity or interference between mouse anti-GPC antibody and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.47U/ml -30 U/ml. Sensitivity: The minimum detectable dose of mouse anti-GPC antibody is typically less than 0.15 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with GPC. Standards and samples are pipetted into the wells with a biotin-conjugated GPC and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of anti-GPC antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells