catalog number :
MBS706771
products type :
ELISA Kit
products full name :
Human RNA polymerase III, Pol III antibody ELISA Kit
products short name :
RNA polymerase III (Pol III) antibody
products name syn :
Human RNA polymerase III (Pol III) antibody ELISA kit; RNA polymerase III (Pol III) antibody
other names :
RNA polymerase III 128kD subunit; DNA-directed RNA polymerase III subunit RPC2; CG8344 gene product from transcript CG8344-RA; CG8344-PA; RNA polymerase III; RNA polymerase III 128kD subunit; RNA polymerase IIIb; RpIII128-PA; RNA polymerase III 128kD subunit; DNA-directed RNA polymerase III 128 kDa polypeptide; C128
other gene names :
RpIII128; RpIII128; CG8344; DmelCG8344; DmRP128; RP128; RP128; CG8344; RNA polymerase III subunit C2; C128
uniprot entry name :
RPC2_DROME
specificity :
This assay has high sensitivity and excellent specificity for detection of human RNA polymerase III (Pol III) antibody. No significant cross-reactivity or interference between human RNA polymerase III (Pol III) antibody and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human RNA polymerase III (Pol III) antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_523706.1
ncbi gb acc num :
NM_078982.3
ncbi mol weight :
128,515 Da
ncbi pathways :
Cytosolic Sensors Of Pathogen-associated DNA Pathway (1005454); Eukaryotic Transcription Initiation Pathway (198256); Gene Expression Pathway (1006408); Immune System Pathway (1005382); Innate Immune System Pathway (1005411); Metabolic Pathways (132021); Purine Metabolism Pathway (2982); Purine Metabolism Pathway (307); Pyrimidine Metabolism Pathway (2984); Pyrimidine Metabolism Pathway (309)
uniprot summary :
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft . Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
size4 :
10x96-Strip-Wells