catalog number :
MBS706711
products type :
ELISA Kit
products full name :
Fish Insulin-like growth factor II, IGF2 ELISA Kit
products short name :
insulin-like growth factor 2 (somatomedin A)
products name syn :
Fish Insulin-like growth factor II (IGF2) ELISA kit; C11orf43; FLJ22066; FLJ44734; INSIGF; pp9974; OTTHUMP00000011018; OTTHUMP00000011157; insulin-like growth factor 2; insulin-like growth factor II; insulin-like growth factor type 2; putative insulin-like; insulin-like growth factor 2 (somatomedin A)
other names :
insulin-like growth factor II preproprotein; Insulin-like growth factor II; insulin-like growth factor II; insulin growth factor 2
products gene name :
IGF2
other gene names :
IGF2; IGF2; IGF-II; IGF-II
uniprot entry name :
IGF2_SHEEP
specificity :
This assay has high sensitivity and excellent specificity for detection of Fish IGF2. No significant cross-reactivity or interference between Fish IGF2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 62.5 pg/ml-1000 pg/ml. Sensitivity: 31.25 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: glyconeogenesis and glycometabolism. Protein Biological Process 3: Carbohydrate metabolism
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF2. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated IGF2. The competitive inhibition reaction is launched between with HRP-conjugated IGF2 and IGF2 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of IGF2 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001009311.1
ncbi gb acc num :
NM_001009311.1
ncbi mol weight :
19,616 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
size4 :
10x96-Strip-Wells