catalog number :
MBS706472
products type :
ELISA Kit
products full name :
Human Glutamate decarboxylase 1, GAD1 autoantibody ELISA Kit
products short name :
Glutamate decarboxylase 1 (GAD1) autoantibody
products name syn :
Human Glutamate decarboxylase 1 (GAD1) autoantibody ELISA kit ; Human Glutamate decarboxylase 1 (GAD1) autoantibody
other names :
glutamate decarboxylase 1; Glutamate decarboxylase 1; glutamate decarboxylase 1
products gene name :
GAD1
other gene names :
GAD; GAD1; GAD1; glutamate decarboxylase; GLUTAMATE DECARBOXYLASE; GLUTAMATE DECARBOXYLASE 1; MKP11.30; MKP11_30; GAD; GDH1; At5g17330; MKP11.30; MKP11_18; GAD 1
uniprot entry name :
DCE1_ARATH
specificity :
This assay has high sensitivity and excellent specificity for detection of human GAD1 autoantibody. No significant cross-reactivity or interference between human GAD1 autoantibody and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<20%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human GAD1 autoantibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_197235.1
ncbi gb acc num :
NM_121739.3
ncbi mol weight :
57,066 Da
ncbi pathways :
Alanine, Aspartate And Glutamate Metabolism Pathway (100103); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Butanoate Metabolism Pathway (4062); Butanoate Metabolism Pathway (391); Metabolic Pathways (132014); Taurine And Hypotaurine Metabolism Pathway (4028); Taurine And Hypotaurine Metabolism Pathway (336); Beta-Alanine Metabolism Pathway (4027); Beta-Alanine Metabolism Pathway (335); Glutamate Degradation IV Pathway (546168)
uniprot summary :
Function: Catalyzes the production of GABA. The calmodulin-binding is calcium-dependent and it is proposed that this may, directly or indirectly, form a calcium regulated control of GABA biosynthesis.1 PublicationManual assertion based on experiment in:Ref.5. Catalytic activity: L-glutamate = 4-aminobutanoate + CO2. Cofactor: Pyridoxal phosphate. Enzyme regulation: Up-regulatd by calmodulin binding at physiological pH.1 PublicationManual assertion based on experiment in:Ref.10
size3 :
10x96-Strip-Wells